Curd J G, Sundsmo J S, Kolb W P, Bluestein H G, Müller-Eberhard H J
Arthritis Rheum. 1978 Mar;21(2):177-82. doi: 10.1002/art.1780210201.
Since complement activation and hematological abnormalities occur in systemic lupus erythematosus (SLE), the present study is an investigation of whether the membrane attack complex of complement might be bound to peripheral blood leukocytes (PBL) in vivo. Assembly of the membrane attack complex results in the generation of neoantigen (neoAg) which is complex-specific and not expressed by any of the individual complement proteins. FITC antiserum specific to neoAg was employed to detect the membrane attack complex on PBL from 7 normal donors, 12 patients with SLE, and 2 patients with rheumatoid arthritis (RA): 3 +/- 1% of normal, 25 +/- 13% of SLE, and 23 +/- 11% of RA PBL were positive. The majority of the neoAg positive PBL in SLE were polymorphonuclear neutrophils (PMN) as shown by adherence to plastic, phagocytosis of carbonyl iron, and differential cell counts. The PBL were greater than 98% viable as indicated by the trypan blue exclusion technique. These observations strongly suggest that the membrane attack complex may be bound to viable PBL in patients with SLE and RA, and further raise the possibility that the membrane attack complex, may have a function other than lysis.
由于系统性红斑狼疮（SLE）中会发生补体激活和血液学异常，本研究旨在调查补体膜攻击复合物在体内是否可能与外周血白细胞（PBL）结合。膜攻击复合物的组装导致新抗原（neoAg）的产生，该新抗原具有复合物特异性，且任何单个补体蛋白均不表达。使用针对neoAg的异硫氰酸荧光素（FITC）抗血清来检测7名正常供体、12名SLE患者和2名类风湿性关节炎（RA）患者的PBL上的膜攻击复合物：正常PBL的阳性率为3±1%，SLE患者为25±13%，RA患者为23±11%。如通过塑料贴壁、羰基铁吞噬和细胞分类计数所示，SLE中大多数neoAg阳性PBL为多形核中性粒细胞（PMN）。锥虫蓝排斥技术表明，PBL的活力大于98%。这些观察结果强烈表明，膜攻击复合物可能与SLE和RA患者的存活PBL结合，并进一步增加了膜攻击复合物可能具有除溶解作用以外的其他功能的可能性。
