Mergenhagen S E, Notkins A L, Evans R T
Immunology. 1966 Sep;11(3):223-8.
The influence of various concentrations of human γ-globulin (HGG) employed to sensitize tanned sheep erythrocytes on haemagglutination titres with various early and late mouse and rabbit antisera to HGG has been studied. The concentration of HGG employed to sensitize tanned erythrocytes which gives the highest haemagglutination titres with early, mercaptoethanol-sensitive antibodies was not optimal for obtaining highest haemagglutination titres with late, mercaptoethanol-insensitive antibodies. Similar results were obtained with heavy and light sucrose gradient ultracentrifugation fractions of a late and early rabbit antiserum. These findings may account for past discrepancies and should be considered when employing the haemagglutination test to detect early and late antibodies.
研究了用不同浓度的人γ-球蛋白（HGG）致敏鞣酸处理的绵羊红细胞，对不同早期和晚期小鼠及兔抗HGG血清血凝滴度的影响。用于致敏鞣酸红细胞的HGG浓度，与早期巯基乙醇敏感抗体产生最高血凝滴度时，对于获得晚期巯基乙醇不敏感抗体的最高血凝滴度并非最佳。早期和晚期兔抗血清的重蔗糖梯度和轻蔗糖梯度超速离心级分也得到了类似结果。这些发现可能解释了过去的差异，在采用血凝试验检测早期和晚期抗体时应予以考虑。
