Cohn Z A, Parks E
J Exp Med. 1967 Jun 1;125(6):1091-104. doi: 10.1084/jem.125.6.1091.
Bovine sera contain factors which are capable of agglutinating mouse erythrocytes and stimulating the pinocytic activity of cultivated mouse macrophages. The hemagglutinating and vesicle-inducing activities of sera increase with the age of the animal and are absent in fetal calf serum. The majority of this material is recovered in globulin fractions prepared with Na(2)SO(4)-(NH(4))(2)SO(4) and is absent in bovine fraction II. It behaves as a macroglobulin in studies employing zone electrophoresis, Sephadex G-200 filtration, sucrose density gradient centrifugation, and in its sensitivity to 2-mercaptoethanol and heat. Absorption of bovine sera with either mouse erythrocytes or spleen cells removes the hemagglutinating and pinosome-inducing properties of the sera. The addition of small quantities of bovine macroglobulin to mouse macrophages results in a stimulation of pinocytic activity, phase-dense granule formation and the cellular content of three acid hydrolases. In the presence of heat-labile factors, the macroglobulin initiates the hemolysis of mouse erythrocytes and the cytolysis of mouse macrophages. This material is thought to represent an interspecies gammaM-type antibody directed against common antigenic determinants on the mouse erythrocyte and macrophage surface.
牛血清中含有能够凝集小鼠红细胞并刺激培养的小鼠巨噬细胞吞噬活性的因子。血清的血凝活性和诱导囊泡活性随动物年龄增长而增加，胎牛血清中不存在这些活性。这种物质的大部分存在于用Na₂SO₄-(NH₄)₂SO₄制备的球蛋白组分中，而在牛组分II中不存在。在采用区带电泳、Sephadex G - 200过滤、蔗糖密度梯度离心的研究中，以及在其对2 - 巯基乙醇和热的敏感性方面，它表现为一种巨球蛋白。用小鼠红细胞或脾细胞吸收牛血清可去除血清的血凝和诱导吞噬体的特性。向小鼠巨噬细胞中添加少量牛巨球蛋白会刺激吞噬活性、相致密颗粒形成以及三种酸性水解酶的细胞含量。在存在热不稳定因子的情况下，巨球蛋白会引发小鼠红细胞的溶血和小鼠巨噬细胞的细胞溶解。这种物质被认为代表一种针对小鼠红细胞和巨噬细胞表面共同抗原决定簇的种间γM型抗体。
