兔脾脏细胞培养物中脱氧核糖核酸合成的抗原刺激的进一步研究。I. 离心蛋白质的作用。
Further studies of antigen stimulation of deoxyribonucleic acid synthesis in rabbit spleen cell cultures. I. The effects of centrifuged proteins.
作者信息
Harris G, Cramp W A
出版信息
Immunology. 1968 Mar;14(3):409-14.
Abstract
The rate of DNA synthesis, as measured by the uptake of [C]thymidine by spleen cell suspensions from rabbits previously immunized to heterologous proteins, was specifically stimulated by the deposits obtained after high-speed centrifugation of solutions of these antigens. The deposited material was much more stimulatory than the original protein or the supernatants from centrifuged preparations. Recombination of the centrifuged deposit with the supernatant resulted in restoration of the lost stimulatory activity.
摘要
用[C]胸苷摄取量来衡量DNA合成速率，对于先前已免疫过异种蛋白的兔脾细胞悬液而言，这些抗原溶液经高速离心后获得的沉淀物能特异性地刺激其DNA合成速率。沉淀物质的刺激作用比原始蛋白质或离心制剂的上清液要强得多。将离心沉淀物与上清液重新组合，会使丧失的刺激活性得以恢复。
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