Bloch-Shtacher N, Hirschhorn K, Uhr J W
Clin Exp Immunol. 1968 Nov;3(9):889-99.
The stimulatory effect of antigen–antibody–complement complexes on cultured normal human peripheral blood lymphocytes was studied. The donors of the cells had not been sensitized to the antigens used. Two antigens were used: flagellar antigen of B (SPB) and bovine serum albumin (BSA), with their respective antibodies prepared in rabbits. The addition of such antigen–antibody aggregates to the cultures stimulated the lymphocytes as determined by morphological changes and increased uptake of [C]thymidine into DNA. Peak of stimulation was observed after 5–6 days of culture incubation. The stimulation appeared to be complement dependent. The lymphocytes showed no response either to the antigen alone or to anti-SPB. When BSA–anti-BSA was centrifuged, most of the stimulatory activity was found in the supernate. The most likely explanation of this stimulation is injury to lymphocyte membranes, possibly from a non-specific attachment of immune complexes to them. A similar mechanism of membrane injury may underlie reactions to all non-specific stimulants, and possibly also to specific antigens to which the cell donor is sensitized.
研究了抗原 - 抗体 - 补体复合物对培养的正常人外周血淋巴细胞的刺激作用。细胞供体未对所用抗原致敏。使用了两种抗原：B型副伤寒杆菌鞭毛抗原（SPB）和牛血清白蛋白（BSA），并分别用兔制备了它们各自的抗体。将此类抗原 - 抗体聚集体添加到培养物中，通过形态学变化以及[C]胸苷掺入DNA量增加来确定，刺激了淋巴细胞。培养孵育5 - 6天后观察到刺激峰值。这种刺激似乎依赖补体。淋巴细胞对单独的抗原或抗SPB均无反应。当牛血清白蛋白 - 抗牛血清白蛋白离心后，大部分刺激活性存在于上清液中。这种刺激最可能的解释是淋巴细胞膜受到损伤，可能是由于免疫复合物与其非特异性结合。类似的膜损伤机制可能是对所有非特异性刺激物反应的基础，也可能是细胞供体致敏的特异性抗原反应的基础。
