Hsu L, Trupin G L
Virchows Arch B Cell Pathol. 1978 Mar 2;27(1):49-61. doi: 10.1007/BF02888982.
C1300 neuroblastoma was implanted with regenerating skeletal muscle to study the role of tissue interactions during tumor cell differentiation. Combined tumor-muscle implants, placed subcutaneously or within diffusion chambers were compared with control tumors implanted without muscle. Neuroblastoma implanted with injured muscle undergoes a partial neuronal differentiation. The tumor cells lose their normal round cell configuration and develop numerous cytoplasmic processes. Accompanying these outward changes are an increased content of microtubules in the neuritic processes; the appearance of glial-like processes containing abundant microfilaments; and the occurrence of growth vesicles identical to those of the growth cones of normal neurites. Although the implants usually contain large numbers of regenerated myofibers, tumor cell differentiation is not dependent upon the presence of these newly formed fibers. Tumor differentiation occurs equally well on the surfaces of degenerating muscle fragments, fibrin deposits and on the membrane surfaces of the diffusion chambers. These observations suggest that non-specific cell surface phenomena, rather than neuromuscular interactions were primarily responsible for the tumor cell differentiation in vivo.
将C1300神经母细胞瘤与再生骨骼肌一起植入，以研究肿瘤细胞分化过程中组织相互作用的作用。将皮下或扩散室内植入的肿瘤 - 肌肉联合植入物与未植入肌肉的对照肿瘤进行比较。植入受损肌肉的神经母细胞瘤会发生部分神经元分化。肿瘤细胞失去其正常的圆形细胞形态，并长出许多细胞质突起。伴随这些外在变化的是神经突中微管含量增加；出现含有丰富微丝的胶质样突起；以及出现与正常神经突生长锥相同的生长小泡。尽管植入物通常含有大量再生肌纤维，但肿瘤细胞的分化并不依赖于这些新形成纤维的存在。肿瘤在退化的肌肉碎片表面、纤维蛋白沉积物以及扩散室的膜表面同样能很好地分化。这些观察结果表明，非特异性细胞表面现象而非神经肌肉相互作用是体内肿瘤细胞分化的主要原因。
