Overton J
J Cell Biol. 1969 Jan;40(1):136-43. doi: 10.1083/jcb.40.1.136.
Lanthanum staining of embryonic chick cell reaggregates reveals an intercellular material composed of fibrils. Fibrillar arrays may be composed of parallel fibrils with a 35 A center-to-center spacing. Fibrils may also be disoriented, long, and tortuous. Newly dissociated cells show little lanthanum staining surface material, but appreciable amounts are present after 6 hr of reaggregation. Examination of intact tissue does not give the same clear evidence of a fibrillar matrix surrounding the cells, but treatment with a number of agents permits observation of intercellular fibrils, and in some cases there is evidence of orientation. Thus fibrillar material must be taken into account in considering mechanisms of cell aggregation.
对鸡胚细胞重聚体进行镧染色,可揭示出一种由纤维组成的细胞间物质。纤维阵列可能由中心间距为35埃的平行纤维组成。纤维也可能是无定向的、长而弯曲的。刚解离的细胞几乎没有镧染色表面物质,但重聚6小时后会出现相当数量的镧染色表面物质。对完整组织的检查并没有提供同样明确的证据表明细胞周围存在纤维状基质,但用多种试剂处理后可以观察到细胞间纤维,在某些情况下还有取向的证据。因此,在考虑细胞聚集机制时必须考虑纤维状物质。