Diena B B, Ashton F E, Ryan A, Wallace R
Can J Microbiol. 1978 Feb;24(2):117-23. doi: 10.1139/m78-022.
The ability of R-type lipopolysaccharide (LPS), isolated from Neisseria gonorrhoeae colony type 4, to protect against infection with N. gonorrhoea colony type 1 (T1 isolates) in the mouse and chicken embryo was investigated. C57 black mice were immunized intraperitoneally with 50 microgram of LPS, and challenged intracerebrally with 10-20 LD50's of N. gonorrhoeae colony type 1. Immunized mice were significantly protected (P less than 0.01 to less than 0.05) against challenge with different T1 isolates of N. gonorrhoeae when compared with non-immunized mice. Mice, injected with succinylated or alkali-treated LPS were not protected against gonococcal challenges. In a second animal model, leghorn hens were immunized intravenously with three injections of 500 microgram of LPS followed by a booster of 2.5 mg 2 weeks later. Embryonated eggs obtained from immunized hens were protected against challenge with 5 x 10(3) - 1 x 10(4) LD50's of three different T1 isolates. When hens were injected with the chemically modified LPS, the embryos were not resistant to gonococcal challenge. The results of this study demonstrate the ability of R-type gonococcal LPS to provide protection against different T1 isolates of N. gonorrhoeae.
研究了从淋病奈瑟菌4型菌落中分离出的R型脂多糖（LPS）在小鼠和鸡胚中抵御1型淋病奈瑟菌（T1分离株）感染的能力。将50微克LPS腹腔注射免疫C57黑小鼠，然后用10 - 20个1型淋病奈瑟菌半数致死量进行脑内攻击。与未免疫小鼠相比，免疫小鼠在面对不同的1型淋病奈瑟菌T1分离株攻击时受到显著保护（P小于0.01至小于0.05）。注射琥珀酰化或碱处理LPS的小鼠未受到淋病奈瑟菌攻击的保护。在第二个动物模型中，给来航鸡静脉注射三次500微克LPS，两周后再注射2.5毫克进行加强免疫。从免疫母鸡获得的受精蛋在面对三种不同T1分离株的5×10³ - 1×10⁴个半数致死量攻击时受到保护。当给母鸡注射化学修饰的LPS时，胚胎对淋病奈瑟菌攻击无抵抗力。本研究结果表明R型淋病奈瑟菌LPS有能力抵御不同的1型淋病奈瑟菌T1分离株。
