在淋病奈瑟菌血清学诊断试验中核酸酶增强特异性细胞凝集作用
Nuclease enhancement of specific cell agglutination in a serodiagnostic test for Neisseria gonorrhoeae.
作者信息
Arko R J, Wong K H, Peacock W L
出版信息
J Clin Microbiol. 1979 Apr;9(4):517-9. doi: 10.1128/jcm.9.4.517-519.1979.
Abstract
Antiserum to a purified type R lipopolysaccharide antigen isolated from Neisseria gonorrhoeae was used in a slide agglutination test and compared with conventional carbohydrate utilization and fluorescent antibody tests to confirm the identity of laboratory cultures classified as typical or "atypical" N. gonorrhoeae. Cultures of Corynebacterium vaginalis, N. meningitidis, N. catarrhalis, N. sicca, and N. lactamicus were also tested in the slide agglutination procedure. The addition of both deoxyribonuclease and ribonuclease (1 mg/ml) to the cell suspension medium of phosphate-buffered saline improved the sensitivity and specificity of the agglutination reaction for N. gonorrhoeae. Problems relating to the agglutination test as an aid in identification of N. gonorrhoeae are discussed.
摘要
从淋病奈瑟菌中分离出的纯化R型脂多糖抗原的抗血清用于玻片凝集试验，并与传统的碳水化合物利用试验和荧光抗体试验进行比较，以确认分类为典型或“非典型”淋病奈瑟菌的实验室培养物的身份。阴道棒状杆菌、脑膜炎奈瑟菌、卡他莫拉菌、干燥奈瑟菌和产乳酸奈瑟菌的培养物也用玻片凝集法进行了检测。在磷酸盐缓冲盐水的细胞悬浮培养基中加入脱氧核糖核酸酶和核糖核酸酶（1mg/ml）可提高淋病奈瑟菌凝集反应的敏感性和特异性。讨论了凝集试验在淋病奈瑟菌鉴定中的相关问题。
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