Rajewsky K, Schirrmacher V, Nase S, Jerne N K
J Exp Med. 1969 Jun 1;129(6):1131-43. doi: 10.1084/jem.129.6.1131.
Rabbits primarily stimulated with a BSA (bovine serum albumin)-sulfanilic acid complex will produce a good secondary response to the sulfanilic acid hapten if the carrier used in the secondary stimulus is again BSA, and not if the secondary carrier is HGG (human gamma globulin). In the latter situation, a good secondary response is obtained, however, if the rabbits are pretreated a few weeks earlier with free HGG. We conclude that the immune stimulus involves the recognition of carrier determinants unrelated to the hapten. As the receptors for recognition of unrelated determinants are probably situated on different cells, we suggest that the immune stimulus leading to antibody formation requires the interaction of two antigen-bridged cells.
主要用牛血清白蛋白（BSA）-磺胺酸复合物刺激的兔子，如果再次刺激时使用的载体是BSA，那么它们会对磺胺酸半抗原产生良好的二次反应；而如果二次刺激的载体是人类丙种球蛋白（HGG），则不会产生这种反应。然而，在后一种情况下，如果兔子在几周前用游离的HGG进行预处理，就会获得良好的二次反应。我们得出结论，免疫刺激涉及对与半抗原无关的载体决定簇的识别。由于识别无关决定簇的受体可能位于不同的细胞上，我们认为导致抗体形成的免疫刺激需要两个抗原桥接细胞的相互作用。
