Ford W L, Simonsen M
J Exp Med. 1971 Apr 1;133(4):938-49. doi: 10.1084/jem.133.4.938.
Using a popliteal lymph node weight assay the graft-versus-host activity of lymphocytes from donors immunized with allogeneic tissue has been assayed by comparison with that of lymphocytes from nonimmune donors. When the donors were immunized against weak histocompatibility antigens (non-AgB) the specific GVH activity of its lymphocytes was increased. This increase was greater if spleen cells rather than thoracic duct lymphocytes were the source of the donor cells used for assay. The increase in GVH activity was also greater if the standard immunization procedure of two successive skin allografts was followed by three boosting injections of allogeneic lymphoid cells. When donors were immunized against strong histocompatibility antigens the specific GVH activity of the donors' lymphocytes was slightly increased, was unchanged, or was actually decreased depending on the experimental situation. In donors rendered incapable of a humoral alloantibody response by whole body X-irradiation, immunization across a strong barrier was followed by little or no increase in the specific GVH activity of TDL. In the rat, as in other species, the increase in GVH activity after immunization is inversely proportional to the strength of the antigenic barrier involved.
采用腘窝淋巴结重量测定法，通过与非免疫供体淋巴细胞的活性进行比较，对用同种异体组织免疫的供体淋巴细胞的移植物抗宿主活性进行了测定。当供体针对弱组织相容性抗原（非AgB）进行免疫时，其淋巴细胞的特异性移植物抗宿主活性增加。如果用于测定的供体细胞来源是脾细胞而非胸导管淋巴细胞，则这种增加更为明显。如果在连续两次皮肤同种异体移植的标准免疫程序之后，再进行三次同种异体淋巴细胞的加强注射，移植物抗宿主活性的增加也会更大。当供体针对强组织相容性抗原进行免疫时，根据实验情况，供体淋巴细胞的特异性移植物抗宿主活性会略有增加、保持不变或实际上降低。在通过全身X射线照射而无法产生体液同种异体抗体反应的供体中，跨越强屏障进行免疫后，胸导管淋巴细胞的特异性移植物抗宿主活性几乎没有增加或根本没有增加。在大鼠中，与其他物种一样，免疫后移植物抗宿主活性的增加与所涉及的抗原屏障强度成反比。
