Slowey R R, Eidelman S, Klebanoff S J
J Bacteriol. 1968 Sep;96(3):575-9. doi: 10.1128/jb.96.3.575-579.1968.
The peroxidase of human parotid saliva has been purified by concentration, gel filtration on Sephadex G-200, and ion exchange chromatography on Amberlite CG-50. The purified product was devoid of amylase activity, lysozyme activity, and immunoglobulin A (IgA). However, it had an inhibitory effect on the growth of Lactobacillus acidophilus in complete growth medium and on lysine accumulation by L. acidophilus in a buffer-glucose medium, when combined with thiocyanate ions. The concentrations of peroxidase and thiocyanate ions employed were within the range found in saliva. The fractions which contained IgA did not have an anti-bacterial effect on L. acidophilus under the conditions employed. Parotid saliva also contained low molecular weight inhibitors of peroxidase activity. These studies support the involvement of the salivary peroxidase in an antibacterial system in saliva.
人腮腺唾液中的过氧化物酶已通过浓缩、在葡聚糖凝胶G - 200上进行凝胶过滤以及在Amberlite CG - 50上进行离子交换色谱法进行了纯化。纯化后的产物不含淀粉酶活性、溶菌酶活性和免疫球蛋白A（IgA）。然而，当与硫氰酸根离子结合时，它对嗜酸乳杆菌在完全生长培养基中的生长以及嗜酸乳杆菌在缓冲葡萄糖培养基中赖氨酸的积累具有抑制作用。所使用的过氧化物酶和硫氰酸根离子的浓度在唾液中发现的范围内。在所采用的条件下，含有IgA的组分对嗜酸乳杆菌没有抗菌作用。腮腺唾液还含有过氧化物酶活性的低分子量抑制剂。这些研究支持唾液过氧化物酶参与唾液中的抗菌系统。
