Smith P B, Hancock G A, Rhoden D L
Appl Microbiol. 1969 Dec;18(6):991-3. doi: 10.1128/am.18.6.991-993.1969.
Incorporation of methyl green dye into an agar medium containing deoxyribo-nucleic acid results in an improved medium for detecting deoxyribonuclease-producing bacteria. Use of the dye makes it unnecessary to use acid to demonstrate deoxyribonuclease activity, thus allowing subculture or reincubation of colonies. The improved medium is highly sensitive and can be used for primary isolation of Staphylococcus aureus or group A Streptococcus pyogenes.
将甲基绿染料掺入含有脱氧核糖核酸的琼脂培养基中，可得到一种用于检测产脱氧核糖核酸酶细菌的改良培养基。使用该染料无需用酸来证明脱氧核糖核酸酶的活性，从而可对菌落进行传代培养或再次培养。这种改良培养基高度敏感，可用于金黄色葡萄球菌或A组化脓性链球菌的初次分离。
