The effect of antisera against rat immunoglobulins on cellular transfer of experimental allergic encephalomyelitiin Lewis rats.

Lymph node cell (LNC) transfers of experimental allergic encephalomyelitis (EAE) in Lewis rats were performed in which suspensions of sensitized LNC, prior to injection into recipients, were incubated with antisera against rat whole serum or rat γ-globulin. No interference with the transfer of EAE was observed. Fluorescent antibody studies revealed essentially no binding of anti-rat globulin to suspensions of intact LNC. Definite binding of anti-rat globulin was demonstrable, however, after alcohol-fixation of LNC. These observations provide additional evidence that immune responses of the delayed-type intimately associated with sensitized lymph node cell suspensions are more important than conventional immunoglobulin responses in development of EAE.