Mickenberg I D, Root R K, Wolff S M
J Clin Invest. 1970 Aug;49(8):1528-38. doi: 10.1172/JCI106370.
The phagocytic, bactericidal, and metabolic capabilities of circulating blood leukocytes from three adults (two males, one female) with hypogammaglobulinemia and recurrent pneumonia, chronic sinusitis, and intestinal giardiasis were studied. These functions were found to be normal when leukocytes from the patients were incubated in media containing normal human serum. Phagocytosis of Staphylococcus albus and polystyrene balls by both patient and normal leukocytes was diminished when the cells were incubated in hypogammaglobulinemic plasma. A similar defect in opsonization by patient plasma was also noted for pneumococci, Escherichia coli and variably with Staphylococcus aureus. Both patient and normal sera had equivalent levels of heat-labile S. albus opsonins; normal serum, however, contained heat-stable S. albus-specific absorbable opsonins in significantly greater quantities to account for its superior opsonic capacity. The addition of commercial gamma globulin or purified IgG to hypogammaglobulinemic sera restored full S. albus opsonic activity. The relevancy of these observations to the impaired host defenses in these patients will be discussed.
对三名患有低丙种球蛋白血症且反复发生肺炎、慢性鼻窦炎和肠道贾第虫病的成年人（两名男性，一名女性）循环血白细胞的吞噬、杀菌和代谢能力进行了研究。当将患者的白细胞在含有正常人血清的培养基中孵育时，发现这些功能正常。当细胞在低丙种球蛋白血症血浆中孵育时，患者和正常白细胞对白色葡萄球菌和聚苯乙烯球的吞噬作用均减弱。对于肺炎球菌、大肠杆菌以及对金黄色葡萄球菌的调理作用存在一定差异，患者血浆的调理作用也存在类似缺陷。患者和正常血清中热不稳定的白色葡萄球菌调理素水平相当；然而，正常血清中含有大量热稳定的白色葡萄球菌特异性可吸收调理素，以解释其更强的调理能力。向低丙种球蛋白血症血清中添加商业γ球蛋白或纯化的IgG可恢复白色葡萄球菌的完全调理活性。将讨论这些观察结果与这些患者宿主防御受损的相关性。
