Trelstad R L
J Cell Biol. 1970 Apr;45(1):34-42. doi: 10.1083/jcb.45.1.34.
The intracellular position of the Golgi apparatuses in the basal cell layer of the corneal epithelium in embryonic and hatched chicks has been studied in the light microscope by impregnating the Golgi apparatus with silver. During two distinct periods in development the Golgi apparatuses in the basal cells shift from an apical to basal position. Each of these periods correlates in time with the appearance of an acellular collagenous matrix beneath the epithelium. Examination of the basal epithelial cells in the electron microscope confirms the intracellular shifts in position of the Golgi apparatus. The results suggest that the Golgi apparatus shifts to the basal cell pole of the corneal epithelium in order to excrete connective tissue materials into the developing corneal stroma.
利用银浸染高尔基器,通过光学显微镜研究了胚胎期和刚孵出雏鸡角膜上皮基底细胞层中高尔基器的细胞内位置。在发育的两个不同时期,基底细胞中的高尔基器从顶端位置转移至基底位置。这两个时期中的每个时期在时间上都与上皮下方无细胞胶原基质的出现相关。电子显微镜对基底上皮细胞的检查证实了高尔基器在细胞内位置的转移。结果表明,高尔基器转移至角膜上皮的基底细胞极,以便将结缔组织物质分泌到正在发育的角膜基质中。