Dooley D C, Nester E W
J Bacteriol. 1973 May;114(2):711-22. doi: 10.1128/jb.114.2.711-722.1973.
The fate of transforming deoxyribonucleic acid (DNA) in Bacillus subtilis was studied by isolating the DNA-membrane complex on Renografin gradients. Soon after uptake, transforming DNA binds to the cell membrane and displays a greater resistance to shear than the recipient genome-membrane complex. Ten minutes after uptake, a portion of the donor DNA is released from the membrane. Most of the released donor radioactivity represents unintegrated, biologically inactive DNA. Recombinant or integrated DNA is enriched 1.5- to 1.7-fold in the membrane. This enrichment last at least 30 min after termination of DNA uptake, and probably much longer. The data suggest that transforming DNA may be integrated into the recipient genome on, or close to, the cell membrane.
通过在泛影葡胺梯度上分离DNA-膜复合物，研究了枯草芽孢杆菌中转化脱氧核糖核酸（DNA）的命运。摄取后不久，转化DNA与细胞膜结合，并且比受体基因组-膜复合物对剪切力具有更大的抗性。摄取后十分钟，一部分供体DNA从膜上释放出来。释放的供体放射性大部分代表未整合的、无生物活性的DNA。重组或整合的DNA在膜中富集了1.5至1.7倍。这种富集在DNA摄取终止后至少持续30分钟，可能持续更长时间。数据表明，转化DNA可能在细胞膜上或其附近整合到受体基因组中。
