Reilly B E, Zeece V M, Anderson D L
J Virol. 1973 May;11(5):756-60. doi: 10.1128/JVI.11.5.756-760.1973.
With bacteriophage phi29 of Bacillus subtilis 133, suppressor-sensitive (sus) hydroxylamine mutants have been isolated. Intracistronic and intercistronic quantitative complementation placed the mutants in 13 cistrons, and three-factor crosses have been used to assign an unambiguous order for 10 cistrons. Recombination frequencies have been presented for several regions of the genome to facilitate comparison of the sus system with the previously published temperature-sensitive mapping systems.
利用枯草芽孢杆菌133的噬菌体phi29,已分离出对抑制因子敏感(sus)的羟胺突变体。顺反子内和顺反子间的定量互补分析将这些突变体定位到13个顺反子中,并且已通过三因子杂交为10个顺反子确定了明确的顺序。已给出基因组几个区域的重组频率,以便于将sus系统与先前发表的温度敏感作图系统进行比较。