枯草芽孢杆菌噬菌体phi 29的遗传分析：两个菌株库参考突变体的整合与定位
Genetic analysis of bacteriophage phi 29 of Bacillus subtilis: integration and mapping of reference mutants of two collections.
作者信息
Mellado R P, Moreno F, Viñuela E, Salas M, Reilly B E, Anderson D L
出版信息
J Virol. 1976 Aug;19(2):495-500. doi: 10.1128/JVI.19.2.495-500.1976.
Abstract
Reference mutants of Bacillus subtilis phage phi 29 of the Madrid and Minneapolis collections were employed to construct a genetic map. Suppressor-sensitive and temperature-sensitive mutants were assigned to 17 cistrons by quantitative complementation. Three-factor crosses were used to assign an unambiguous order for the 17 cistrons. Recombination frequencies determined by two-factor crosses were used to construct a linear genetic map of 24.4 recombination units. The genes were numbered sequentially from left to right (1 to 17) according to their relative map position.
摘要
马德里和明尼阿波利斯菌株收藏的枯草芽孢杆菌噬菌体 phi 29 的参考突变体被用于构建遗传图谱。通过定量互补将抑制敏感和温度敏感突变体分配到17个顺反子。利用三因子杂交为这17个顺反子确定明确的顺序。由双因子杂交确定的重组频率被用于构建一个24.4重组单位的线性遗传图谱。根据基因的相对图谱位置，从左到右依次编号（1至17）。
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