血红素在哺乳动物蛋白质合成中的作用：一种起始因子的激活
Role for heme in mammalian protein synthesis: activation of an initiation factor.
作者信息
Raffel C, Stein S, Kaempfer R
出版信息
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4020-24. doi: 10.1073/pnas.71.10.4020.
Abstract
Two nonerythropoietic tissues, brian and liver, contain an initiation factor that can overcome the block in initiation of protein synthesis seen in reticulocyte lysates when exogenous hemin is not present. Upon incubation of the brain factor with hemin and removal of free hemin by gel filtration, the factor activity is strongly stimulated. This stimulation shows a concentration dependence on hemin close to that seen for stimulation of protein synthesis in whole reticulocyte lysates. The data indicate that hemin mediates the formation of an active initiation factor complex from inactive, lower molecular weight components.
摘要
两种非红细胞生成组织，即脑和肝脏，含有一种起始因子，在外源血红素不存在时，该因子能克服在网织红细胞裂解物中所见的蛋白质合成起始受阻现象。将脑因子与血红素一起温育并通过凝胶过滤去除游离血红素后，该因子活性受到强烈刺激。这种刺激显示出对血红素的浓度依赖性，与在整个网织红细胞裂解物中刺激蛋白质合成时所见的情况相近。数据表明，血红素介导了由无活性的、较低分子量成分形成活性起始因子复合物的过程。
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本文引用的文献
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Proc Natl Acad Sci U S A. 1968 Feb;59(2):582-9. doi: 10.1073/pnas.59.2.582.
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Control of globin synthesis: the role of heme.珠蛋白合成的调控：血红素的作用。
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