Santo L, Leighton T J, Doi R H
J Bacteriol. 1973 Aug;115(2):703-6. doi: 10.1128/jb.115.2.703-706.1973.
Morphological studies of a conditionally temperature-sensitive ribonucleic acid polymerase mutant of Bacillus subtilis have revealed that sporulation is inhibited at stage II when the cells are grown at 47.5 C. Growth and sporulation occur normally at 30 C with the mutant. The mutant grows normally at 47.5 C but is prevented from sporulating at the nonpermissive temperature by an abnormal septation during forespore membrane formation which prevents the subsequent engulfment process (stage III). The mutation affects the normal functioning of ribonucleic acid polymerase at the nonpermissive temperature resulting in abortive sporulation.
对枯草芽孢杆菌的一种条件性温度敏感核糖核酸聚合酶突变体的形态学研究表明，当细胞在47.5℃下生长时，芽孢形成在第二阶段受到抑制。该突变体在30℃下正常生长和形成芽孢。该突变体在47.5℃下正常生长，但在非允许温度下，前芽孢膜形成过程中的异常隔膜阻止了随后的吞噬过程（第三阶段），从而使其无法形成芽孢。该突变在非允许温度下影响核糖核酸聚合酶的正常功能，导致芽孢形成失败。
