Santo L, Leighton T J, Doi R H
J Bacteriol. 1972 Jul;111(1):248-53. doi: 10.1128/jb.111.1.248-253.1972.
The morphological characteristics of wild-type Bacillus subtilis and a temperature-sensitive serine protease derivative have been observed during vegetative and sporulation time periods. At 30 C wild-type and mutant cells grow and sporulate identically. At 47.5 C wild-type and mutant cells grow identically, but the mutant cells are blocked at stage 0 or I in the sporulation sequence. Wild-type cells sporulate normally at 47.5 C.
在营养生长和芽孢形成阶段,对野生型枯草芽孢杆菌及其温度敏感型丝氨酸蛋白酶衍生物的形态特征进行了观察。在30℃时,野生型和突变型细胞的生长和芽孢形成情况相同。在47.5℃时,野生型和突变型细胞的生长情况相同,但突变型细胞在芽孢形成序列的0期或I期受阻。野生型细胞在47.5℃时能正常形成芽孢。