Leighton T
J Biol Chem. 1977 Jan 10;252(1):268-72.
A single site mutant of Bacillus subtilis with a streptovaricin-resistant RNA polymerase has been isolated; this mutation caused temperature-sensitive sporulation, but had no effect on vegetative growth. The mutant (ts710) temperature-sensitive period irreversibly affected the middle and late stages of sporulation. Mutant cells grown at the nonpermissive temperature exhibited abnormal serine protease accumulation, serine esterase accumulation, alkaline phosphatase accumulation, RNA polymerase template specificity changes, and pulse-labeled RNA synthesis profiles. The accumulation of metal protease was not affected at the nonpermissive temperature. Attempts to isolate single site mutants which were streptolydigin-resistant, and temperature-sensitive for sporulation, were unsuccessful.
已分离出具有抗链黑菌素RNA聚合酶的枯草芽孢杆菌单一位点突变体；该突变导致温度敏感型芽孢形成，但对营养生长没有影响。突变体（ts710）的温度敏感期不可逆地影响芽孢形成的中期和后期。在非允许温度下生长的突变体细胞表现出异常的丝氨酸蛋白酶积累、丝氨酸酯酶积累、碱性磷酸酶积累、RNA聚合酶模板特异性变化以及脉冲标记的RNA合成谱。在非允许温度下，金属蛋白酶的积累不受影响。尝试分离对链霉溶菌素具有抗性且对芽孢形成温度敏感的单一位点突变体，但未成功。
