枯草芽孢杆菌无芽孢突变体中核糖核酸聚合酶的模板特异性
Template specificity of ribonucleic acid polymerase in asporogenous mutants of Bacillus subtilis.
作者信息
Brevet J, Sonenshein A L
出版信息
J Bacteriol. 1972 Dec;112(3):1270-4. doi: 10.1128/jb.112.3.1270-1274.1972.
Abstract
Asporogenous mutants of Bacillus subtilis were examined for the change in template specificity of ribonucleic acid (RNA) polymerase characteristic of wild-type cells undergoing sporulation. Mutants blocked at stages II, III, and IV showed a changed specificity of the enzyme after the end of growth and were in this respect indistinguishable from the wild type. The RNA polymerase of eight stage-zero mutants (out of nine tested) which possess mutations that map at six distinct loci retained the template specificity of vegetative cells.
摘要
对枯草芽孢杆菌的无芽孢形成突变体进行了检查，以观察其核糖核酸（RNA）聚合酶模板特异性的变化，这种变化是野生型细胞在形成芽孢过程中所特有的。在II、III和IV阶段受阻的突变体在生长结束后显示出该酶的特异性发生了变化，在这方面与野生型无法区分。九个测试的零阶段突变体中有八个（其突变位于六个不同位点）的RNA聚合酶保留了营养细胞的模板特异性。
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