枯草芽孢杆菌溴化乙锭抗性突变体
Ethidium bromide-resistant mutant of Bacillus subtilis.
作者信息
Bishop P E, Brown L R
出版信息
J Bacteriol. 1973 Sep;115(3):1077-83. doi: 10.1128/jb.115.3.1077-1083.1973.
Abstract
An ethidium bromide-resistant mutant (EB8) derived from a Marburg strain of Bacillus subtilis was found to be conditionally resistant to 10 mug of ethidium bromide per ml. Expression of resistance is complete only during vegetative growth at incubation temperatures above 30 C in complex medium or minimal medium supplemented with Casamino Acids. Strain EB8 is cross-resistant to acriflavine and proflavine. The ethidium bromide resistance marker is co-transduced with hisA1 at a frequency of 6% and is located to the right of hisA1 on the B. subtilis chromosome as it is usually represented on the map. Incorporation of [5-(3)H] uridine by strain EB8 showed that ribonucleic acid synthesis in both whole cells and protoplasts is ethidium bromide-resistant.
摘要
从枯草芽孢杆菌马尔堡菌株衍生出的一种耐溴化乙锭突变体（EB8）被发现对每毫升10微克溴化乙锭具有条件抗性。只有在复合培养基或添加了酪蛋白氨基酸的基本培养基中，于30℃以上的培养温度下进行营养生长时，抗性表达才完全。EB8菌株对吖啶黄和原黄素具有交叉抗性。溴化乙锭抗性标记与hisA1共转导，频率为6%，并且按照通常在图谱上的表示，它位于枯草芽孢杆菌染色体上hisA1的右侧。EB8菌株对[5-(3)H]尿苷的掺入表明，全细胞和原生质体中的核糖核酸合成对溴化乙锭具有抗性。
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