Bryner J H, Ritchie A E, Booth G D, Foley J W
Appl Microbiol. 1973 Sep;26(3):404-9. doi: 10.1128/am.26.3.404-409.1973.
Five phages isolated from lysogenic strains of Vibrio fetus var. venerealis and two from V. fetus var. intestinalis were tested for lytic activity on 95 V. fetus strains from various animal and human hosts. In addition, virion and plaque morphology of the seven phages were compared. Electron micrographs showed that all were the kite-tailed variety with minor variations in head and tail dimensions. Plaques of V45 and V2 were small, clear and irregular; those of V3, V8, and V19 were large, clear and regular at the edge; the plaques of V16 and V20 were intermediate in size, clear, and very irregular at the edge with satellite plaques. The number of strains lysed by one or more phages were as follows: 29 of 30 from cattle; 7 of 11 from sheep; 1 of 5 from pigs; 1 of 1 from a monkey; and 33 of 42 from human hosts. Four natural groups of phages were derived by statistical measures of percentage of similarity in lytic activity. Group III lysed more strains (46 of 95) than any of the others. Twenty-five strains were lysed by group IV, 23 strains by group I, and 19 strains by group II. Results of this study indicate that phage typing should be a practical supplement to other differential tests for V. fetus.
对从胎儿弧菌性病变种的溶原性菌株中分离出的5株噬菌体以及从胎儿弧菌肠道变种中分离出的2株噬菌体，检测了它们对来自各种动物和人类宿主的95株胎儿弧菌的裂解活性。此外，还比较了这7株噬菌体的病毒体和噬菌斑形态。电子显微镜照片显示，所有噬菌体均为风筝尾型，头部和尾部尺寸略有差异。V45和V2的噬菌斑小、清晰且不规则；V3、V8和V19的噬菌斑大、清晰且边缘规则；V16和V20的噬菌斑大小中等、清晰，边缘非常不规则且有卫星噬菌斑。被一种或多种噬菌体裂解的菌株数量如下：来自牛的30株中有29株；来自羊的11株中有7株；来自猪的5株中有1株；来自一只猴子的1株全部被裂解；来自人类宿主的42株中有33株。通过对裂解活性相似性百分比的统计测量得出了4个噬菌体自然组。第三组裂解的菌株（95株中的46株）比其他任何一组都多。第四组裂解了25株，第一组裂解了23株，第二组裂解了19株。本研究结果表明，噬菌体分型应是胎儿弧菌其他鉴别试验的一种实用补充。
