Iguchi T, Takasugi N
Anat Embryol (Berl). 1979 Jan 30;155(2):127-34. doi: 10.1007/BF00305746.
Estrogen-independent, persistent proliferation and cornification of the vaginal epithelium occurred in ovariectomized adult mice which had received neonatal injections of 50 microgram 5alpha-dihydrotestosterone (DHT). The occurrence of the permanent vaginal changes was prevented by injections of 200 IU vitamin A acetate (VA) given simultaneously with DHT. In 45% of the DHT plus VA-treated, ovariectomized mice, clear cells with pale cytoplasm appeared in the basal layer of the degenerating epithelium, while they came out in the epithelium of 13% of the ovariectomized mice receiving neonatal injections of DHT alone. For suppressing the permanent vaginal changes, including high mitotic rate, more than 1 month was required after the combined treatment. However, the occurrence of the permanent changes was not blocked by VA given after the DHT treatment. Permanent proliferation and squamous metaplasia took place in the uterine epithelium of the DHT-treated mice. Neonatal injections of VA also prevented the occurrence of the permanent uterine changes when given simultaneously with DHT but not when given after the androgen treatment.
在新生期注射过50微克5α-二氢睾酮（DHT）的成年去卵巢小鼠中，出现了不依赖雌激素的阴道上皮持续增殖和角质化现象。与DHT同时注射200国际单位醋酸维生素A（VA）可防止永久性阴道变化的发生。在45%接受DHT加VA治疗的去卵巢小鼠中，退化上皮基底层出现了细胞质淡染的透明细胞，而在仅接受新生期DHT注射的13%去卵巢小鼠的上皮中也出现了这种细胞。为抑制包括高有丝分裂率在内的永久性阴道变化，联合治疗后需要1个多月的时间。然而，DHT治疗后给予VA并不能阻止永久性变化的发生。DHT处理的小鼠子宫上皮发生了永久性增殖和鳞状化生。新生期注射VA与DHT同时给予时也可防止永久性子宫变化的发生，但在雄激素治疗后给予则无效。
