Karamata D, McConnell M, Rogers H J
J Bacteriol. 1972 Jul;111(1):73-9. doi: 10.1128/jb.111.1.73-79.1972.
Nine class A salt-dependent rod mutants were mapped on the Bacillus subtilis genome by PBS1-mediated transduction. They are distributed into two small linkage groups designated rod B and rod C; mutations in rod B are over 80% cotransducible with pheA and different mutations in rod C are 12 to 21% cotransducible with hisA. It is established that neither rod B nor rod C is linked by transformation to the other identified rod mutations present in 168-ts-200B and 8332 glu(-). It is hypothesized that salt-dependent mutations are due to enzyme alterations which are corrected by high salt concentrations.
通过PBS1介导的转导,在枯草芽孢杆菌基因组上定位了九个A类盐依赖性杆状突变体。它们被分为两个小的连锁群,分别命名为rod B和rod C;rod B中的突变与pheA的共转导率超过80%,rod C中的不同突变与hisA的共转导率为12%至21%。已确定,rod B和rod C通过转化均不与168-ts-200B和8332 glu(-)中存在的其他已鉴定的杆状突变相连锁。据推测,盐依赖性突变是由于酶的改变,而高盐浓度可纠正这种改变。