Lindgren V, Rutberg L
J Bacteriol. 1974 Aug;119(2):431-42. doi: 10.1128/jb.119.2.431-442.1974.
Bacillus subtilis mutants unable to catabolize glycerol (Glp mutants) were isolated and mapped. The location of the mutations on the chromosome was determined by a density transfer technique and confirmed by PBS1 transduction and transformation. The different mutations were ordered relative to each other. Mutations rendering the cells glycerol auxotrophic were also mapped and found not to be linked to the Glp mutations.
分离并定位了不能分解甘油的枯草芽孢杆菌突变体(Glp突变体)。通过密度转移技术确定了染色体上突变的位置,并通过PBS1转导和转化进行了确认。确定了不同突变之间的相对顺序。还对使细胞成为甘油营养缺陷型的突变进行了定位,发现它们与Glp突变不连锁。