细菌鞭毛的合成。II. 枯草芽孢杆菌中鞭毛特异性标记的PBS1转导
Synthesis of bacterial flagella. II. PBS1 transduction of flagella-specific markers in Bacillus subtilis.
作者信息
Grant G F, Simon M I
出版信息
J Bacteriol. 1969 Jul;99(1):116-24. doi: 10.1128/jb.99.1.116-124.1969.
The linkage relationship of mutants involved in the synthesis of flagella was determined by PBSl transduction. Mutants that affect the structure of flagellin (hag) and temperature-sensitive mutants (flaTS) that produce flagella when grown at 37 C but not when grown at 46 C were examined. All of the mutants were found to be linked to the hisA1 marker. The flaTS mutants fell into three clusters. Group A contained the majority of mutants which were loosely grouped around the hag locus. Group B mutants were segregated from the hag locus and appeared closely linked to the phage adsorption site gene (gtaA), and group C was only loosely linked to hisA1 and thus far contains only one mutant. A flagella locus (ifm) affecting both the degree of motility and level of flagellation was shown to map near group A. Mutants affecting motility (mot) were not linked to hisA1 by PBSl transduction. Several markers previously shown to link to hisA1 were ordered with respect to hisA1 and the flagellar genes.
通过PBS1转导确定了参与鞭毛合成的突变体之间的连锁关系。研究了影响鞭毛蛋白结构的突变体（hag）以及温度敏感突变体（flaTS），后者在37℃生长时能产生鞭毛，但在46℃生长时则不能。所有突变体均被发现与hisA1标记连锁。flaTS突变体分为三个簇。A组包含大多数突变体，它们松散地聚集在hag基因座周围。B组突变体与hag基因座分离，似乎与噬菌体吸附位点基因（gtaA）紧密连锁，而C组仅与hisA1松散连锁，到目前为止只包含一个突变体。一个影响运动程度和鞭毛形成水平的鞭毛基因座（ifm）被证明定位于A组附近。影响运动性的突变体（mot）通过PBS1转导不与hisA1连锁。几个先前显示与hisA1连锁的标记相对于hisA1和鞭毛基因进行了排序。
Zotero 插件