通过顶空气相色谱法检测变形杆菌属物种从蛋氨酸产生甲硫醇和二甲基二硫的情况。
Methyl mercaptan and dimethyl disulfide production from methionine by Proteus species detected by head-space gas-liquid chromatography.
作者信息
Hayward N J, Jeavons T H, Nicholson A J, Thornton A G
出版信息
J Clin Microbiol. 1977 Sep;6(3):187-94. doi: 10.1128/jcm.6.3.187-194.1977.
Abstract
Head-space gas-liquid chromatography and mass spectrometry were used to detect and identify products formed by Proteus vulgaris, P. mirabilis, P. morganii, and P. rettgeri from a defined medium supplemented with either phenylalanine, methionine, valine, leucine, histidine, lysine, ornithine, threonine, asparagine, aspartic acid, or tryptophan. In a detailed study of the products formed by 68 strains of Proteus spp. from L-methionine, the production of large amounts of both dimethyl disulfide and methyl mercaptan was found to be a characteristic of the genus. Both sulfur products appeared within a few hours of inoculation. Dimethyl disulfide was a more sensitive indicator of growth than the spectrometric determination of optical density. This suggests that it could be useful for the rapid, automated detection of any species of Proteus.
摘要
采用顶空气相色谱和质谱法检测和鉴定普通变形杆菌、奇异变形杆菌、摩根氏变形杆菌和雷氏变形杆菌在添加苯丙氨酸、蛋氨酸、缬氨酸、亮氨酸、组氨酸、赖氨酸、鸟氨酸、苏氨酸、天冬酰胺、天冬氨酸或色氨酸的特定培养基中形成的产物。在一项对68株变形杆菌属菌株由L-蛋氨酸形成的产物的详细研究中，发现大量产生二甲基二硫和甲硫醇是该属的一个特征。两种含硫产物在接种后数小时内出现。二甲基二硫比光密度的光谱测定更能灵敏地指示生长情况。这表明它可用于快速、自动检测任何变形杆菌属菌种。
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Methyl mercaptan and dimethyl disulfide production from methionine by Proteus species detected by head-space gas-liquid chromatography.通过顶空气相色谱法检测变形杆菌属物种从蛋氨酸产生甲硫醇和二甲基二硫的情况。
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