Hayward N J, Jeavons T H, Nicholson A J, Thornton A G
J Clin Microbiol. 1977 Sep;6(3):187-94. doi: 10.1128/jcm.6.3.187-194.1977.
Head-space gas-liquid chromatography and mass spectrometry were used to detect and identify products formed by Proteus vulgaris, P. mirabilis, P. morganii, and P. rettgeri from a defined medium supplemented with either phenylalanine, methionine, valine, leucine, histidine, lysine, ornithine, threonine, asparagine, aspartic acid, or tryptophan. In a detailed study of the products formed by 68 strains of Proteus spp. from L-methionine, the production of large amounts of both dimethyl disulfide and methyl mercaptan was found to be a characteristic of the genus. Both sulfur products appeared within a few hours of inoculation. Dimethyl disulfide was a more sensitive indicator of growth than the spectrometric determination of optical density. This suggests that it could be useful for the rapid, automated detection of any species of Proteus.
采用顶空气相色谱和质谱法检测和鉴定普通变形杆菌、奇异变形杆菌、摩根氏变形杆菌和雷氏变形杆菌在添加苯丙氨酸、蛋氨酸、缬氨酸、亮氨酸、组氨酸、赖氨酸、鸟氨酸、苏氨酸、天冬酰胺、天冬氨酸或色氨酸的特定培养基中形成的产物。在一项对68株变形杆菌属菌株由L-蛋氨酸形成的产物的详细研究中，发现大量产生二甲基二硫和甲硫醇是该属的一个特征。两种含硫产物在接种后数小时内出现。二甲基二硫比光密度的光谱测定更能灵敏地指示生长情况。这表明它可用于快速、自动检测任何变形杆菌属菌种。
