Hranueli D, Piggot P J, Mandelstam J
J Bacteriol. 1974 Sep;119(3):684-90. doi: 10.1128/jb.119.3.684-690.1974.
As an alternative to exhaustive mapping, an attempt has been made to obtain a rough estimate of the total number of sporulation operons by statistical treatment. Sixteen sporulation mutants taken at random were characterized biochemically and morphologically. The mutations they contained were mapped to determine whether they fell into any one of 23 known operons. From the proportion that do so (10/16), it is calculated that the most probable number of sporulation operons is 37 (68% confidence limits of 31 and 46). If allowance is made for the fact that two of the operons apparently contain mutagenic "hot spots" and the calculation is amended accordingly, the most probable numbers of operons becomes 42 (limits 33 and 59).
作为详尽图谱绘制的替代方法,已尝试通过统计处理对芽孢形成操纵子的总数进行粗略估计。随机选取了16个芽孢形成突变体,对其进行了生化和形态学特征分析。确定了它们所含突变的图谱,以判断这些突变是否属于23个已知操纵子中的任何一个。根据归入已知操纵子的比例(10/16)计算得出,芽孢形成操纵子最可能的数量为37个(68%置信区间为31至46)。如果考虑到其中两个操纵子明显含有诱变“热点”这一事实并相应修正计算,则操纵子最可能的数量变为42个(区间为33至59)。