利用温度敏感型芽孢形成突变体对枯草芽孢杆菌中的芽孢形成操纵子进行遗传定位。
Genetic mapping of sporulation operons in Bacillus subtilis using a thermosensitive sporulation mutant.
作者信息
Young M
出版信息
J Bacteriol. 1975 Jun;122(3):1109-16. doi: 10.1128/jb.122.3.1109-1116.1975.
Abstract
A thermosensitive sporulation mutant was used to determine the order of sporulation operonsin the urs region of the Bacillus subtilis chromosome. Data from three-factor transformation crosses and three- and four-factor transduction crosses established the order metC-SPO-96(SpoII)-spo-85(SpoV)-spo-279(SpoII)-furA-ura-cysC-spo-NG1.67(SpoIII). Previously, furA was thought to lie to the right of ura and cysC to the left (Dubnau, 1970; Young and Wilson, 1972).
摘要
利用一个温度敏感型芽孢形成突变体来确定枯草芽孢杆菌染色体urs区域中芽孢形成操纵子的顺序。三因子转化杂交以及三因子和四因子转导杂交的数据确定了以下顺序：metC-SPO-96（SpoII）-spo-85（SpoV）-spo-279（SpoII）-furA-ura-cysC-spo-NG1.67（SpoIII）。此前，furA被认为位于ura的右侧，而cysC位于左侧（杜布瑙，1970年；扬和威尔逊，1972年）。
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