Hsu T C, Arrighi F E, Klevecz R R, Brinkley B R
J Cell Biol. 1965 Aug;26(2):539-53. doi: 10.1083/jcb.26.2.539.
In a number of mammalian cell strains nucleoli persisted through mitosis. This phenomenon was especially pronounced in several cell lines derived from Chinese hamster tissues. All the methods employed, including radioautography with tritiated uridine, cytochemical stains (methyl green-pyronin and azure B), fluorescent microscopy (coriphosphine O), ribonuclease digestion, and electron microscopy, demonstrated that the bodies identified as persistent nucleoli in the mitotic stages had the same characteristics as did the nucleoli in the interphase. Persistent nucleoli may attach to the chromosomes or may be free in the cytoplasm. In cells where no persistent nucleoli as such were noted, nucleolar material was observed to attach to the chromosomes in shapeless masses which moved with the chromosomes during anaphase. At least a portion of the nucleolar material was included in the daughter nuclei, presumably for immediate use for protein synthesis after cell division.
在许多哺乳动物细胞株中，核仁在有丝分裂过程中持续存在。这种现象在源自中国仓鼠组织的几种细胞系中尤为明显。所采用的所有方法，包括用氚标记的尿苷进行放射自显影、细胞化学染色（甲基绿-派洛宁和天青B）、荧光显微镜检查（考里磷光素O）、核糖核酸酶消化以及电子显微镜检查，都表明在有丝分裂阶段被鉴定为持续存在的核仁的物体与间期核仁具有相同的特征。持续存在的核仁可能附着于染色体，也可能游离于细胞质中。在未观察到此类持续存在核仁的细胞中，观察到核仁物质以无定形团块附着于染色体，在后期随染色体移动。至少一部分核仁物质被纳入子核，大概是为细胞分裂后立即用于蛋白质合成。
