Hubbard R W, Criss R E, Elliott L P, Kelly C, Matthew W T, Bowers W D, Leav I, Mager M
J Appl Physiol Respir Environ Exerc Physiol. 1979 Feb;46(2):334-9. doi: 10.1152/jappl.1979.46.2.334.
A total of 171 untrained, unacclimatized, and unanesthetized rats were either exercised to exhaustion at one of four ambient temperatures (5, 20, 26, or 30 degrees C), or were restrained and heated at an ambient temperature of 41.5 degrees C until their core temperatures reached a preselected end point between 41.0 and 43.3 degrees C. The serum levels of creatine phosphokinase (CRK) and two transaminases (SGOT and SGPT) were determined at 30 min, 24, 48, 72, and 96 h posttreatment. Peak enzyme activity for CPK was noted primarily at the 30-min sampling period and at 24 h for the transaminases. The data indicated that under these conditions a) the transaminase SGOT was elevated in the serum as a consequence of the extent and duration of prior hyperthermia, b) the transaminase SGOT was released in moderate amounts after exhaustive exercise but reached its greatest activity levels following hyperthermia, and c) the activity of CPK was increased by the duration of exhaustive exercise and was less sensitive than either transaminase to prior hyperthermia. As a result, each of the three experimental conditions: a) exercise without hyperthermia, b) exercise with hyperthermia, and c) sedentary hyperthermia, produced a unique pattern of serum enzyme activity that would appear useful in diagnosing a variety of heat- and/or work-induced disorders.
总共171只未经训练、未适应环境且未麻醉的大鼠，要么在四种环境温度（5、20、26或30摄氏度）之一的条件下运动至 exhaustion，要么在环境温度41.5摄氏度下被束缚并加热，直到其核心温度达到41.0至43.3摄氏度之间的预先选定终点。在处理后30分钟、24、48、72和96小时测定血清肌酸磷酸激酶（CRK）和两种转氨酶（SGOT和SGPT）的水平。CPK的酶活性峰值主要在30分钟采样期出现，转氨酶则在24小时出现。数据表明，在这些条件下：a）由于先前高温的程度和持续时间，血清中转氨酶SGOT升高；b）在力竭运动后，转氨酶SGOT有中等量释放，但在高温后达到其最大活性水平；c）CPK的活性因力竭运动的持续时间而增加，并且比任何一种转氨酶对先前高温的敏感性更低。因此，三种实验条件：a）无高温的运动，b）有高温的运动，以及c）久坐不动的高温，各自产生了一种独特的血清酶活性模式，这似乎有助于诊断各种由热和/或工作引起的疾病。
