牛鼻中隔蛋白-多糖复合物的组成与理化性质
The composition and physicochemical properties of bovine nasal-septa protein-polysaccharide complex.
作者信息
Luscombe M, Phelps C F
出版信息
Biochem J. 1967 Jan;102(1):110-9. doi: 10.1042/bj1020110.
Abstract
- Protein-polysaccharide complexes were prepared in three different ways and the gross stoicheiometry of the complexes compared. 2. The neutral sugar content was ascertained and the possibility of a glycoprotein occurring with chondroitin sulphate and keratosulphate is discussed. 3. Physical data support a molecule of molecular weight 3.2x10(6)-5.8x10(6) with a roughly spherical domain and an average radius of gyration of 1390A. Such a particle is highly solvated. The complex is heavily charged with the sulphate groups on the outside. 4. These findings are discussed in the light of the physiological role of protein-polysaccharide light fraction (PPL) in cartilage.
摘要
- 以三种不同方式制备了蛋白质 - 多糖复合物，并比较了这些复合物的总体化学计量。2. 确定了中性糖含量，并讨论了与硫酸软骨素和硫酸角质素一起出现糖蛋白的可能性。3. 物理数据支持分子量为3.2×10⁶ - 5.8×10⁶的分子，其具有大致球形结构域，平均回转半径为1390埃。这样的粒子高度溶剂化。该复合物外部带有大量硫酸根基团。4. 根据蛋白质 - 多糖轻组分（PPL）在软骨中的生理作用对这些发现进行了讨论。
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