Tucci S M, Skalko R G
J Environ Pathol Toxicol. 1979 Jan-Feb;2(3):625-32.
Pregnant albino mice were administered (ip) embryotoxic doses of three individual teratogens: bromodeoxyuridine, hydroxyurea and mitomycin C on day 10 of gestation. Embryos were removed 4 hr later, a cell suspension was prepared and cultured in the presence of colcemid. Metaphase chromosome spreads were subjected to standard G-banding procedures, and the occurrence and frequency of lateral asymmetry (unequal banding of sister chromatids) was monitored. Embryotoxic levels of all three teratogens produced an increase in the number of asymmetries/karotype. For one of these (mitomycin C), the observed increase was dose-dependent.
在妊娠第10天，给怀孕的白化小鼠腹腔注射三种致畸剂（溴脱氧尿苷、羟基脲和丝裂霉素C）的胚胎毒性剂量。4小时后取出胚胎，制备细胞悬液并在秋水仙酰胺存在下进行培养。中期染色体铺展进行标准G显带程序，监测侧向不对称（姐妹染色单体带型不等）的发生情况和频率。所有三种致畸剂的胚胎毒性水平均导致不对称/核型数量增加。对于其中一种（丝裂霉素C），观察到的增加是剂量依赖性的。
