检测子宫内暴露于丝裂霉素C的小鼠胚胎染色体C带中的侧向不对称性。
Detection of lateral asymmetry in the C-bands of mouse-embryo chromosomes exposed in utero to mitomycin C.
作者信息
Tucci S M, Kaye G I
出版信息
Mutat Res. 1980 Aug;72(1):115-8. doi: 10.1016/0027-5107(80)90226-2.
Chromosomes from 10-day mouse embryos were C-banded and examined for aymmetric bands between sister chromatids. No occurrences of lateral asymmetry (LA) were observed in this series. However, when 10-day embryos were exposed in utero to a single maternal injection of 10 mg/kg mitomycin C 4--5 h prior to metaphase arrest with colcemid, a significant level of asymmetric C-banding was demonstrated in their chromosomes. As many as 4 LAs per cell were seen in 2 of the 50 metaphase spreads examined.
对10日龄小鼠胚胎的染色体进行C带染色，并检查姐妹染色单体之间的不对称带。在该系列中未观察到侧向不对称（LA）的情况。然而，当在子宫内用秋水仙酰胺使中期停滞前4 - 5小时给10日龄胚胎的母体单次注射10 mg/kg丝裂霉素C时，其染色体中显示出显著水平的不对称C带。在所检查的50个中期分裂相中，有2个相中每个细胞可见多达4条LA带。
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