铜绿假单胞菌黏液的组成。
Composition of Pseudomonas aeruginosa slime.
作者信息
Brown M R, Foster J H, Clamp J R
出版信息
Biochem J. 1969 May;112(4):521-5. doi: 10.1042/bj1120521.
Abstract
- The slime produced by eight strains of Pseudomonas aeruginosa on a number of different media was demonstrated to be qualitatively the same. Small quantitative differences may be occasioned by differences in the extraction procedure, the growth medium or the strain of organism used. 2. The slime was shown to be predominantly polysaccharide with some nucleic acid material and a small amount of protein. 3. The hydrolysed polysaccharide fraction consists mainly of glucose with smaller amounts of mannose. This accounts for some 50-60% of the total slime. In addition, there is some 5% of hyaluronic acid. The nucleic acid material represents approx. 20% of the total weight, and is composed of both RNA and DNA. 4. Minor components are protein, rhamnose and glucosamine, the protein being less than 5% of the total. 5. Hyaluronic acid is produced in greater quantities from nutrient broth than from chemically defined media, and is more firmly attached to the cells than the other components.
摘要
- 研究表明，8株铜绿假单胞菌在多种不同培养基上产生的黏液在性质上是相同的。提取程序、生长培养基或所用生物体菌株的差异可能会导致少量的定量差异。2. 黏液主要是多糖，含有一些核酸物质和少量蛋白质。3. 水解后的多糖部分主要由葡萄糖组成，还有少量甘露糖。这约占黏液总量的50%-60%。此外，还有约5%的透明质酸。核酸物质约占总重量的20%，由RNA和DNA组成。4. 次要成分是蛋白质、鼠李糖和氨基葡萄糖，蛋白质含量不到总量的5%。5. 营养肉汤中产生的透明质酸比化学限定培养基中产生的更多，并且比其他成分更牢固地附着在细胞上。
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