Mathew C G, Goodwin G H, Johns E W
Nucleic Acids Res. 1979 Jan;6(1):167-79. doi: 10.1093/nar/6.1.167.
Nucleosomes have been isolated from rabbit thymus by sucrose gradient centrifugation, and their high mobility group (HMG) protein content analysed by electrophoresis on polyacrylamide gels. The results suggest that proteins HMG 14 and HMG 17 are associated with the core particle of the nucleosome, and that there are two or more sub-populations of both HMG 1 and HMG 2 molecules. One sub-population appears to be fairly tightly bound to the nucleosome, while another is rapidly released from the chromatin by digestion with micrococcal nuclease. The latter fraction may participate in a higher order folding of the nucleosomes.
已通过蔗糖梯度离心从兔胸腺中分离出核小体，并通过聚丙烯酰胺凝胶电泳分析了它们的高迁移率族（HMG）蛋白含量。结果表明，HMG 14和HMG 17蛋白与核小体的核心颗粒相关，并且HMG 1和HMG 2分子均存在两个或更多个亚群。一个亚群似乎与核小体紧密结合，而另一个亚群通过微球菌核酸酶消化可迅速从染色质中释放出来。后一部分可能参与核小体的高级折叠。
