一种使用羟基磷灰石从染色质中纯化组蛋白对H2A + H2B和H3 + H4的新方法。
A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite.
作者信息
Simon R H, Felsenfeld G
出版信息
Nucleic Acids Res. 1979 Feb;6(2):689-96. doi: 10.1093/nar/6.2.689.
Abstract
A method to purify histone groups H2A+H2B and H3+H4 using dissociation with NaCl and hydroxylapatite chromatography is presented. The procedure is simple, involves mild solvents, and provides milligram quantities of histones of high purity. The histone pairs prepared by this method can regenerate chromatin-like characteristics when combined and reconstituted with DNA.
摘要
介绍了一种使用氯化钠解离和羟基磷灰石色谱法纯化组蛋白H2A + H2B和H3 + H4的方法。该方法简单，使用温和的溶剂，并可提供毫克量的高纯度组蛋白。通过该方法制备的组蛋白对与DNA结合并重构时，能够重现染色质样特征。
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