Sollner-Webb B, Camerini-Otero R D, Felsenfeld G
Cell. 1976 Sep;9(1):179-93. doi: 10.1016/0092-8674(76)90063-5.
We have examined the role by each histone in forming the structure of the nu-body. When DNAase I, DNAase II, trypsin and chymotrypsin attack chromatin, characteristic discrete DNA and protein digest fragments are produced. Using this restriction of accessibility as diagnostic for chromatin structure, we have examined complexes of DNA with virtually all possible combinations of histones. The results strongly support our previous conclusion (Camerini-Otero, Sollner-Webb, and Felsenfeld, 1976) that the arginine-rich histones are unique in their ability to create, with DNA a structure with many features of native chromatin. Acting together, slightly lysine-rich histones then modify this complex into one very similar to native chromatin. An analysis of the rate constants of staphylococcal nuclease digestion also confirms that the complex of H3, H4, and DNA is crucial to the structure of the nu-body.
我们研究了每种组蛋白在形成核小体结构中的作用。当DNA酶I、DNA酶II、胰蛋白酶和胰凝乳蛋白酶作用于染色质时，会产生特征性的离散DNA和蛋白质消化片段。利用这种可及性的限制作为染色质结构的诊断方法，我们研究了DNA与几乎所有可能的组蛋白组合形成的复合物。结果有力地支持了我们之前的结论（卡梅里尼 - 奥泰罗、索尔纳 - 韦伯和费尔森菲尔德，1976年），即富含精氨酸的组蛋白在与DNA形成具有许多天然染色质特征的结构方面具有独特能力。然后，富含赖氨酸的组蛋白共同作用，将这种复合物修饰成一种与天然染色质非常相似的复合物。对葡萄球菌核酸酶消化速率常数的分析也证实，H3、H4和DNA的复合物对核小体的结构至关重要。
