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具有ATP酶活性的哺乳动物微管副晶体的快速纯化及免疫特异性。

Rapid purification and the immunological specificity of mammalian microtubular paracrystals possessing an ATPase activity.

作者信息

Nagayama A, Dales S

出版信息

Proc Natl Acad Sci U S A. 1970 Jun;66(2):464-71. doi: 10.1073/pnas.66.2.464.

DOI:10.1073/pnas.66.2.464
PMID:4247491
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC283068/
Abstract

A new procedure for the rapid purification of vinblastine-induced microtubular paracrystals is described. The paracrystals constitute about 2 per cent of the total protein in L cells. They contain a labile ATPase with a Michaelis constant of 1.0 x 10(-4). In the immunofluorescence test, sera prepared against paracrystals reacted positively with intracellular paracrystals and the mitotic apparatus. Antisera against paracrystals from a malignant murine cell also labeled the mitotic apparatus of HeLa and chicken embryonic cells, revealing the presence of a common or related antigen in the mitotic apparatus of these higher vertebrates.

摘要

本文描述了一种快速纯化长春碱诱导的微管副晶体的新方法。副晶体约占L细胞总蛋白的2%。它们含有一种不稳定的ATP酶，其米氏常数为1.0×10⁻⁴。在免疫荧光试验中，用副晶体制备的血清与细胞内副晶体和有丝分裂器呈阳性反应。来自恶性鼠细胞的副晶体抗血清也标记了HeLa细胞和鸡胚胎细胞的有丝分裂器，揭示了这些高等脊椎动物有丝分裂器中存在共同或相关抗原。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f92/283068/54ed1faa6314/pnas00097-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f92/283068/a9b93c36ea4c/pnas00097-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f92/283068/92f6c2da8eaf/pnas00097-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f92/283068/54ed1faa6314/pnas00097-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f92/283068/a9b93c36ea4c/pnas00097-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f92/283068/92f6c2da8eaf/pnas00097-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f92/283068/54ed1faa6314/pnas00097-0224-a.jpg

相似文献

1
Rapid purification and the immunological specificity of mammalian microtubular paracrystals possessing an ATPase activity.具有ATP酶活性的哺乳动物微管副晶体的快速纯化及免疫特异性。
Proc Natl Acad Sci U S A. 1970 Jun;66(2):464-71. doi: 10.1073/pnas.66.2.464.
2
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Specific visualization of tubulin-containing structures in tissue culture cells by immunofluorescence. Cytoplasmic microtubules, vinblastine-induced paracrystals, and mitotic figures.通过免疫荧光对组织培养细胞中含微管蛋白结构进行特异性可视化。细胞质微管、长春花碱诱导的副晶体和有丝分裂图像。
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J Cell Biol. 1971 Jun;49(3):927-32. doi: 10.1083/jcb.49.3.927.

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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A fine structural study of the formation of temporary swellings (Herring bodies) and reversible degeneration of neurosecretory axons following microiontophoretic ejection of vinblastine into the hypothalamo-neurohypophysial tract of the frog (Rana pipiens).对将长春碱微量离子电渗注入青蛙（豹蛙）下丘脑 - 神经垂体束后神经分泌轴突形成临时肿胀（赫林体）及可逆性变性的精细结构研究。
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Immunologic studies on the influenza A virus nonstructural protein NS1.甲型流感病毒非结构蛋白NS1的免疫学研究
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Immunocytochemistry of the Microtubules of fat-laden cells. Brown fat cells and adrenocortical cells in primary monolayer culture.富含脂肪细胞微管的免疫细胞化学。原代单层培养中的棕色脂肪细胞和肾上腺皮质细胞。
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Synthesis and storage of microtubule proteins by sea urchin embryos.海胆胚胎对微管蛋白的合成与储存
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8
The fine structure and immunological labeling of the achromatic mitotic apparatus after disruption of cell membranes.细胞膜破坏后非染色有丝分裂器的精细结构和免疫标记
J Cell Biol. 1973 Dec;59(3):643-60. doi: 10.1083/jcb.59.3.643.
9
Concerning the universality of a microtubule antigen in animal cells.关于动物细胞中微管抗原的普遍性。
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10
The pathogenesis of epizootic hemorrhagic disease of deer: an electron microscopic study.鹿流行性出血病的发病机制：一项电子显微镜研究。
Am J Pathol. 1973 Mar;70(3):379-400.
Proc Natl Acad Sci U S A. 1963 Aug;50(2):268-75. doi: 10.1073/pnas.50.2.268.
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A negative staining method for high resolution electron microscopy of viruses.一种用于病毒高分辨率电子显微镜观察的负染色方法。
Biochim Biophys Acta. 1959 Jul;34:103-10. doi: 10.1016/0006-3002(59)90237-9.
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Diffusion-in-gel methods for immunological analysis.用于免疫分析的凝胶扩散法。
Prog Allergy. 1958;5:1-78.
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Nutrition needs of mammalian cells in tissue culture.组织培养中哺乳动物细胞的营养需求。
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Fluorescent antibody studies with agents of varicella and herpes zoster propagated in vitro.对水痘和带状疱疹病原体在体外繁殖进行的荧光抗体研究。
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Plaque formation and isolation of pure lines with poliomyelitis viruses.脊髓灰质炎病毒的噬斑形成及纯系分离
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The colchicine-binding protein of mammalian brain and its relation to microtubules.哺乳动物脑内的秋水仙碱结合蛋白及其与微管的关系。
Biochemistry. 1968 Dec;7(12):4466-79. doi: 10.1021/bi00852a043.
10
Microtubule crystals: a new biophysical phenomenon induced by Vinca alkaloids.微管晶体：长春花生物碱诱导的一种新生物物理现象。
Nature. 1968 Jun 22;218(5147):1176-7. doi: 10.1038/2181176a0.