Sugihara H, Yonemitsu N, Ohta K, Miyabara S, Nagayama A
Histochemistry. 1983;79(2):227-36. doi: 10.1007/BF00489784.
Cytoplasmic microtubules of fat-laden cells containing fine lipid droplets, such as brown fat cells and adrenocortical cells, were studied in relation to the metabolism of intracellular lipid. In these cells, the amount and distribution of lipid droplets reflect the state of inherent cellular function. Materials used were primary monolayer culture of fetal rat brown fat cells and that of bovine adrenocortical cells. The method was the immunocytochemistry with anti-tubulin antibody. When brown fat cells were being lipolyzed or the steroidogenesis of adrenocortical cells were being stimulated, the cytoplasmic microtubules in the cells were organized in a radial pattern in response to the behavior of the lipid droplets. It is assumed that the microtubules were in the regulation of cellular function in terms of the metabolism of lipid droplets in these cells. We have devised, in the course of the current study, a double fluorescence technique as an observational method whereby microtubules were observed immunocytochemically and lipid droplets by a secondary fluorescence with phosphine E staining.
对含有细小脂滴的富含脂肪细胞（如棕色脂肪细胞和肾上腺皮质细胞）的细胞质微管进行了研究，探讨其与细胞内脂质代谢的关系。在这些细胞中，脂滴的数量和分布反映了细胞固有功能的状态。所用材料为胎鼠棕色脂肪细胞和牛肾上腺皮质细胞的原代单层培养物。方法是用抗微管蛋白抗体进行免疫细胞化学检测。当棕色脂肪细胞发生脂解或肾上腺皮质细胞的类固醇生成受到刺激时，细胞内的细胞质微管会根据脂滴的行为呈放射状排列。据推测，在这些细胞中，微管在脂滴代谢方面对细胞功能具有调节作用。在本研究过程中，我们设计了一种双重荧光技术作为观察方法，通过免疫细胞化学观察微管，并通过磷光E染色的二次荧光观察脂滴。
