Murison J, Festi D, Ross P E, Bouchier I A
Clin Chim Acta. 1976 Apr 15;68(2):159-66. doi: 10.1016/0009-8981(76)90415-0.
Total and lipid phosphorus were measured in the duodenal aspirate of 24 fasting subjects following an injection of cholecystokinin. The lipid phosphorus values were lower than the total phosphorus, a difference most pronounced in dilute samples. Storage at -20 degrees C over 4 weeks resulted in a loss of over 50% lipid phosphorus. Such alterations in the lipid phosphorus affected the calculation of biliary phospholipid and hence the saturation index of cholesterol in bile causing it to be erroneously elevated. It is concluded that analysis of bile samples should be undertaken on freshly obtained samples and include a preliminary step for the extraction of lipids.
在24名空腹受试者注射胆囊收缩素后，对其十二指肠抽吸物中的总磷和脂质磷进行了测量。脂质磷值低于总磷，这种差异在稀释样本中最为明显。在-20℃下储存4周以上导致脂质磷损失超过50%。脂质磷的这种变化影响了胆汁磷脂的计算，从而影响了胆汁中胆固醇的饱和指数，导致其错误升高。结论是，胆汁样本的分析应采用新鲜获取的样本，并包括脂质提取的初步步骤。
