从牛心脏瓣膜中分离硫酸软骨素及其特性
Isolation and properties of chondroitin sulphates from bovine heart valves.
作者信息
Lowther D A, Preston B N, Meyer F A
出版信息
Biochem J. 1970 Jul;118(4):595-601. doi: 10.1042/bj1180595.
Abstract
- Several protein-polysaccharides were isolated from the soluble extracts of bovine heart valves by sedimentation equilibrium in a caesium chloride density gradient (Meyer, Preston & Lowther, 1969). 2. Compositional and structural studies indicated that the polysaccharide moiety was chondroitin sulphate. Differences in the protein content of the products were observed. There was no evidence suggesting the presence of keratan sulphate. 3. Sedimentation studies indicated that the molecular weights of the samples were between 4.2x10(4) and 6.5x10(4). The results are discussed in terms of a basic model for the protein-polysaccharides of two polysaccharide chains linked by a protein of variable size.
摘要
- 通过在氯化铯密度梯度中进行沉降平衡，从牛心脏瓣膜的可溶性提取物中分离出了几种蛋白质多糖（迈耶、普雷斯顿和洛瑟，1969年）。2. 成分和结构研究表明，多糖部分是硫酸软骨素。观察到产物蛋白质含量存在差异。没有证据表明存在硫酸角质素。3. 沉降研究表明，样品的分子量在4.2×10⁴至6.5×10⁴之间。根据由大小可变的蛋白质连接的两条多糖链的蛋白质多糖基本模型对结果进行了讨论。
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