Liapina L A, Strukova S M, Kudriashov B A
Vopr Med Khim. 1979 Jan-Feb;25(1):41-6.
Formation of complex between heparin and prothrombin was shown using a spectral method. A method is developed for production of active complex heparin-prothrombin with the ratio of components as 1 : 6. The heparin-prothrombin complex obtained exhibited the anticoagulant and antipolymerization properties as well as the nonenzymatic fibrinolytic effect on instabilized fibrin both in absence and in presence of such blocking agents of enzymatic fibrinolysis as epsilon-aminocapronic acid and soybean inhibitor of trypsin. The increase in the anticoagulant and nonenzymatic fibrinolytic properties of blood occurred after intravenous administration of the complex into animals. The heparin-prothrombin complex acted in circulation within 1.5--2 hrs.
采用光谱法证实了肝素与凝血酶原之间复合物的形成。开发了一种制备活性肝素 - 凝血酶原复合物的方法，其组分比例为1:6。所获得的肝素 - 凝血酶原复合物在不存在和存在诸如ε-氨基己酸和大豆胰蛋白酶抑制剂等酶促纤维蛋白溶解阻断剂的情况下，均表现出抗凝、抗聚合特性以及对不稳定纤维蛋白的非酶促纤维蛋白溶解作用。将该复合物静脉注射给动物后，血液的抗凝和非酶促纤维蛋白溶解特性增强。肝素 - 凝血酶原复合物在循环中作用1.5 - 2小时。
