Muller M T, Hudson J B
J Virol. 1977 May;22(2):267-72. doi: 10.1128/JVI.22.2.267-272.1977.
Synchronized murine 3T3 cells have been used to investigate the possible dependency of murine cytomegalovirus replication upon the cell cycle. The normal latent period of 12 h characteristic of asynchronous 3T3 cells was protracted to more than 24 h after an early G1 infection in synchronous cells. In this case viral progeny were not detected until after the initiation of the host S-phase. Cells maintained in the G1 phase did not replicate virus. This failure could not be explained by a decrease in virus penetration but was apparently due to a requirement for an event associated with the host S-phase. Thymidine-induced inhibition of cell cycle traverse also blocked virus replication. Viral DNA synthesis did not initiate until after the initiation of host DNA. In contrast, herpes simplex virus type 1 replicated in 3T3 cells independently of the cell cycle.
同步化的小鼠3T3细胞已被用于研究小鼠巨细胞病毒复制对细胞周期的可能依赖性。异步3T3细胞正常的12小时潜伏期，在同步化细胞中早期G1期感染后延长至超过24小时。在这种情况下，直到宿主S期开始后才检测到病毒后代。维持在G1期的细胞不复制病毒。这种失败不能用病毒穿透减少来解释，显然是由于需要与宿主S期相关的事件。胸腺嘧啶核苷诱导的细胞周期进程抑制也阻断了病毒复制。病毒DNA合成直到宿主DNA开始后才启动。相比之下，1型单纯疱疹病毒在3T3细胞中独立于细胞周期进行复制。
