Pass D A, Thompson R G
Can J Comp Med. 1971 Jul;35(3):181-6.
Studies on the site of proliferation of Pasteurella haemolytica in the bovine nasal cavity have been carried out.P.haemolytica were isolated from 15 selected major anatomical areas of the nasal cavity in calves with high numbers of P.haemolytica following shipment from Western Canada. When the organisms were present in the nasal cavity of live animals in low numbers, they were isolated from many, but not all, areas. P.haemolytica was isolated post mortem from one or more selected areas of several nasal cavities in spite of negative antemortem cultures. By the direct fluorescent antibody technique, P.haemolytica was demonstrated at the surface of nasal epithelial cells. Organisms were not seen in or between epithelial cells nor in the ducts nor alveoli of glands. The findings were similar when high and low numbers of P.haemolytica were present in the nasal cavity.
对溶血巴斯德氏菌在牛鼻腔内的增殖部位进行了研究。从加拿大西部运输后携带大量溶血巴斯德氏菌的犊牛鼻腔15个选定的主要解剖区域分离出了溶血巴斯德氏菌。当活菌鼻腔内的细菌数量较少时，它们能从许多但并非所有区域分离出来。尽管生前培养呈阴性，但死后仍从几个鼻腔的一个或多个选定区域分离出了溶血巴斯德氏菌。通过直接荧光抗体技术，在鼻上皮细胞表面发现了溶血巴斯德氏菌。在上皮细胞内、上皮细胞之间、导管或腺体的腺泡中均未发现细菌。当鼻腔内溶血巴斯德氏菌数量较多和较少时，结果相似。
