Allen J W, Viel L, Bateman K G, Rosendal S, Shewen P E, Physick-Sheard P
Department of Clinical Studies, Ontario Veterinary College, University of Guelph.
Can J Vet Res. 1991 Oct;55(4):341-6.
The upper and lower respiratory tracts of 59 feedlot calves with clinical signs of naturally occurring respiratory disease (cases) and 60 comparison (control) animals were cultured before treatment, using nasopharyngeal swabs (NPS) and bronchoalveolar lavage (BAL). The most prevalent organisms were Pasteurella multocida and Mycoplasma bovis. Isolations of P. multocida from NPS and BAL fluid were found to be significantly associated with morbidity (p less than or equal to 0.05), but the frequency with which other organisms were isolated from the nasopharynx and lungs was similar in cases and controls. There was evidence of moderate agreement between NPS and BAL isolates at the individual calf level using the kappa statistic, (range of kappa values = 0.47-0.61) but the variability of the kappa statistics was large. Therefore, in an individual calf NPS cultures did not accurately predict BAL cultures. The NPS and BAL culture results were quite similar at the group level, however.
对59头有自然发生呼吸道疾病临床症状的育肥牛犊（病例）和60头对照动物的上、下呼吸道在治疗前进行培养，采用鼻咽拭子（NPS）和支气管肺泡灌洗（BAL）。最常见的病原体是多杀性巴氏杆菌和牛支原体。从NPS和BAL液中分离出多杀性巴氏杆菌与发病率显著相关（p≤0.05），但病例组和对照组从鼻咽部和肺部分离出其他病原体的频率相似。使用kappa统计量发现在个体牛犊水平上NPS和BAL分离株之间存在中度一致性（kappa值范围=0.47-0.61），但kappa统计量的变异性很大。因此，在个体牛犊中，NPS培养不能准确预测BAL培养。然而，在组水平上，NPS和BAL培养结果相当相似。
